Development and Application of Polystyrene-based Monolithic Capillary Column and Mass Spectrometric Detection on Capillary Electrophoresis Technique

• Main Authors: Yen-Chen Liu, 劉彥辰
• Other Authors: Hsi-Ya Huang
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/96664138014367405778

碩士 === 中原大學 === 化學研究所 === 95 === In the study, the development and application of Capillary Electrophoresis Technology were divided into two parts. The first part of this study, polymeric monolithic capillary columns of capillary electrochromatography (CEC) were developed and used to separate preservatives in commercial products. This work demostated that the first usage of Sodium 4-vinylbenzenesulfonate (BSA) as charge-carried monomer in CEC column. Effect of polymerization time of poly(styrene-divinylbenzene-4-Vinylbenzenesulfonic acid) on the surface area, morphology and separation efficiency were examined . Buffer pH, ionic concentration and acetonitrile content in mobile phase were examined in order to evaluate properties of separation column and optimize mobile phase for separation. This work demostated that this PS-DVB based monolithic column was feasible and could be also applied to the analytes of acidic compounds. The separation time for seven tested analytes was able to be shorten to 2.5 min when the effective length of the monolithic column was reduced as 10 cm (from the inlet to the detector), this ultrafast separation speed was less found when compared to most CEC report. Compared to that of liquid chromatography, Capillary electrophoresis (CE) has higher separation efficiency and lower usage of solvent. In recent ten years, mass spectrometry (MS) has become a key tool for the characterization molecule. In this study, a CE separation coupled to an ESI-MS technology (CE/ESI-MS) was developed for determination of phenolic acid that are found as commonly secondary metabolites in plant. First, a nonaqueous capillary electrophoresis (NACE) method was used to separate seven phenolic acids, and then MS parameter (sheath liquid composition, sheath liquid flow rate, dry gas flow, nebulizing gas flow) was examined in order to achieve optimal mass sensitivity for each phenolic acids. This study demonstrated that the both detection limit and separation resolution of phenolic acids were enhanced successfully by the coupling of NACE and MS technology.