Preparation and Evaluation of Chitosan/CaSO4/platelet Rich Plasma Microspheres as Dental Implant Material

• Main Authors: Pei-hua Tsai, 蔡佩樺
• Other Authors: Shyh Ming Kuo
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/93381570916861294361

碩士 === 義守大學 === 生物醫學工程學系碩士班 === 95 === Periodontal disease and bone cancer might cause severe alveolus bone loss. One common used therapy to treat this disease is so-called debridement. After that, a bone conductive material is put into defect of alveolus bone to improve the regeneration of bony tissues. Thus, the purpose of the thesis is to prepare chitosan/CaSO4 microspheres, by using a high voltage electrostatic system, to act as dental implant (filling) materials. Besides, platelet gel that enriched growth factors are also used an ingredient to prepare chitosan/CaSO4/PRP microspheres which probably can serve as growth factors slow release carrier (after activated with thrombin). The preliminary results revealed that the chitosan/CaSO4/PRP microspheres are in the range of 322.2 ± 40.8μm ~ 471.6 ± 57.9μm in diameter. The in-trapped growth factors are able to sustained release at 24-hour period test. Three groups of microspheres, chitosan/CaSO4 microspheres (Group A) , chitosan/CaSO4 microspheres mixed with thrombin (Group B), chitosan/CaSO4/PRP microspheres mixed with thrombin (Group C) are prepared and the efficacy of these microspheres in bone regeneration are evaluated in pig oral model. The animal implantation study will be observed by HE stain and Trichrome stain.