| Summary: | 碩士 === 義守大學 === 材料科學與工程學系碩士班 === 95 === For Nanomaterials in biological assays and diagnostics, using quantum dots (Qdots) to overcome the drawbacks of organic fluorescence dye or radioactive labels (tags), such as width of emission, narrow excitation spectrum and optical bleaching. But Qdots upon UV excitation, undergo surface degradation that releases highly toxic Cd2+ ions that may be harmful to biological species such as cells. For the reason, Ru nanoparticles have been selected. The silica shell provides a protective encapsulation around the Ru nanoparticles. The silica shell is not only a biocompatible substance and may be non-toxic,but also makes the toxicity reduced and photostability enhancement. It is provides a biofunctional surface for modification and subsequent a variety of biomolecules (e.g. antibodies, proteins, peptides, DNA, etc.) attachment, will enhance the future to be possible direction of the application.
In this research, Ru nanoparticles were prepared by chemical reduction of RuCl3 by NaBH4 at room temperature，and then modification of the nanoparticle surface by using silane coupling agents (3-aminopropyltrimethoxysilnae, APTES), afterwards forms the Ru/SiO2 nanoparticles were produced via Stober’s method by hydrolysis and co-condensation reaction of tetraethylorthosilicate (TEOS). Ru nanoparticles in this research preparation after the gauging knew its fluorescence effect is not remarkable, after coating the SiO2 discovered its fluorescence intensity distinct enhancement,then through heating processing (400 ℃) also to conducive toward favor enhances the fluorescence intensity.
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