| Summary: | 碩士 === 高雄醫學大學 === 牙醫學研究所碩士班 === 95 === Objective：
Earlier, our research shows that angiotensin converting enzyme(ACE) insertion/deletion(I/D) gene polymorphism is significantly related to oral precanceous lesion. We continue to investigate the connection between ACE I/D gene polymorphism oral cancer patient(experiment group) compared with non-oral mucosal lesion patient(control group), and the relation of severity of oral cancer on betel nut chewing patients. We test the hypothesis that ACE I/D gene polymorphism participates in oral cancer prevalance and disease severity.
Materials & Methods：
Using genomic DNA from 111 patients with oral cancer(most are squamous cell carcinoma), we assessed the ACE I/D gene polymorphism by PCR analysis and compared with a noncancer control population(n = 115)
Results：
ACE genotypes are distributed in patients and control subjects as follows; DD is present in 54(48.7%), ID in 19(17.1%), and in 38(34.2%) patients, and DD in 9(7.8%), ID in 18(15.6%), and in 88(76.6%) healthy subjects repectively(p-value<0.005). In oral cancer patients, trend was observed between angiotensin II genotypes and lymph node metastasis in head and neck region(p-value = 0.05).
Conclusions：
ACE I/D gene polymorphism presents higher frequency in oral cancer patients than in cancer-free subjects. And the further analysis suggests the relation between Ag II and lymph node metastasis. Thus, ACE may influence the pathogenesis and disease progression in oral cancer.
|
|---|
