Suppressor of Cytokine Signaling 1 (SOCS1) Gene in Patients with Rheumatoid Arthritis

• Main Authors: Hua-Zhen Chan, 詹華蓁
• Other Authors: Jeng-Hsien Yen
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/60384671406088396217

碩士 === 高雄醫學大學 === 醫學研究所碩士班 === 95 === Suppressor of Cytokine Signaling1 (SOCS1) is an inhibitor of cytokine signaling and has been shown to act in a classical feedback loop to suppress cytokines expression. The expression of SOCS mRNA is negligible in unstimulated cells, but can be induced rapidly on exposure to some cytokines. SOCS1 also reduces SOCS1 and some cytokines gene expression which induced by cytokines stimulation. SOCS1 proteins can modulate inflammatory response. Therefore, SOCS1 may play an important role in the pathogenesis of RA. One-hundred and eighty-one patients with RA and ninety-six healthy controls were enrolled in this study. Total RNA was extracted form peripheral lymphocytes. The levels of SOCS1 RNA were detected by the method of real-time polymerase chain reaction (real-time PCR). Polymorphisms were determent by PCR/restrict fragment length polymorphism method. The associations between SOCS1 RNA levels and clinical manifestations were also investigated. This study showed that SOCS1 RNA levels in peripheral lymphocytes were significantly higher in patient with RA than in controls (p=0.004). The SOCS1 levels were also related to the disease activity of RA. Patients with high disease activity (DAS28>5.1) have significantly higher SOCS1 level than that patients with low disease activity (DAS28<3.2) (p<0.04). A similar finding could also be found in patients with moderate disease activity (≦3.2 DAS28≦5.1) in comparison with those of low disease activity patients (p<0.04). The SOCS1 level were significantly higher in patient with elevated ESR levels than those of patients with normal ESR levels (p=0.02). We also found that RA patients with bone erosion, which was determined by film x-ray, have significantly lower levels of SOCS1 compare with patients without bone erosion (p=0.04). The HLA-DR genotyping were also related to SOCS1 levels. RA patients with HLA-DR3 (+) have significantly lower SOCS1 levels than that of HLA-DR3 (-) patients (p=0.02). A similar finding could also be found in patients with HLA-DR11 (+) comparison with patient with HLA-DR11 (-) (p=0.04). The polymorphism of SOCS1 promoter was not be demonstrated in Taiwanese patients with RA and healthy control. We establish the SOCS1 quantification standard curve. After experimental result, we obtain a diagram of curves with good linear relations. Therefore use serial dilution standard to evaluate SOCS1 expression is a feasible method. The SOCS1 levels in peripheral lymphocytes were as associated with the disease activity and clinical manifestations of RA. This study showed SOCS1 levels are related to HLA-DR genotyping. However, the SOCS1 levels were not related to the polymorphism of SOCS1 promoter.