Genetic Characterization of Microsatellites and AFLP Markers in Genome of Ducks

• Main Authors: Chang-Wen Huang, 黃章文
• Other Authors: 黃木秋
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/56062888346848546661

博士 === 國立中興大學 === 動物科學系所 === 95 === The purpose of this study was to investigate the genetic imformation of duck genome using microsatellite and amplified fragment length polymorphism (AFLP) marker technique. Genomic DNA was amplified by polymerase chain reaction with multicolored fluorescent dye-labeled primers. The PCR products were size fractionated in capillary gel with ABI PRISM® 3100 Avant Genetic Analyzer. Data were collected and analysed using the softwares of GeneScan and Genotyper. The results of microsatellite markers showed that the polymorphism information content (PIC), the effective number of alleles and heterozygosity for APH01, APH07, APH09 and APH14 in the genomic DNA of brown Tsaiya duck lines of selected (S) and control (C) lines were 0.559/2.372/0.578 and 0.747/4.285/0.767, 0.553/2.453/0.592 and 0.528/2.349/ 0.574, 0.738/4.134/0.758 and 0.701/3.587/0.721, 0.301/1.489/0.329 and 0.265/1.399/0.286. The polymorphisms of microsatellite loci were found in genomic DNA of ducks. There was noticeable genetic variability only for APH01 between lines, able to elaborate the effects of selection and crossbreeding within S line of female Tsaiya ducks breed the different frequency of APH01 198, APH07 200 and APH09 126 allele were resulted in higher performances of average maximum duration of fertility (AMD), fertility rate (FR), hatchability rate (HR) and number of total fertile egg (FECT) traits in both lines. The results of AFLP markers showed that the peaks of polymorphism, reproducible and inheritance could be generated by restriction endonucleases, adapters and primer combinations of TaqI and EcoRI in genomic DNA of ducks. A total of 296 polymorphic peaks were detected by using 18 pairs of fluorescent TaqI/EcoRI primer combinations and each polymorphic marker was analyzed by Chi-square tests. Linkage analysis was performed by CarteBlanche software and linkage maps were drawn by MapChart 2.2 software. The results of AFLP linkage map showed a total of 260 co-dominant markers distributed in 32 linkage groups. There were 3 to 63 molecular markers in each linkage group, which had a range of lengths between 19.9 cM and 171.9 cM. The length of the whole linkage map is 1,766 cM, with an average interval distance between two consecutive markers of 7.75 cM. In conclusion, the results presented in this study show that the different allele frequency of microsatellite loci in both lines were effected by long-term selection to result in the significance of phenotypic traits with fertility rate, hatchability rate, average maximum duration of fertility and average number of total fertile egg. The AFLP fingerprinting might be a suitable method for duck paternity testing. The AFLP linkage map also provided an information for the genetic improvement in ducks.