| Summary: | 碩士 === 中興大學 === 動物科學系所 === 95 === The aim of this study was to establish DNA marker detection systems for single nucleotide polymorphisms associated with economic traits in breeding pigs and genotyping by multiplex minisequencing method, which include genes (CRC, RN and CAST) for meat quality, reproduction (ESR1, PRLR, RBP4, LEP, BF, LEPR, LIF, ACTN1, EPOR, FSHR, GNRHR, PGK2 and RLN), disease resistance (FUT1 and BPI), growth (MC4R and GH), and carcass (PIT1, MYOG and LXRB). Specific primer pairs were designed by GCG sequence analysis software and each of the target genes was amplified. The PCR-RFLP, minisequencing and multiplex-minisequencing methods were used for genotyping. The results showed that the multiplex-minisequencing method was able to unambiguously and simultaneously genotype many SNP loci in different animals. Genotypic and allelic frequencies of each gene in Duorc, Landrace, LD and black pigs were analyzed. It was found there were existence of alleles are favored in MYOG, CAST, BPI (AvaII), BF, ACTN1, PGK2 genes, GH, PIT1, MC4R, PRKAG3, FUT1, BPI (HpaII), LEPR, EPOR, and GNRHR genes still remained room of the genetic improvement in these pig breeds. We have successfully developed a rapid, cost-effective, and high throughput assay for genotyping and can be applied to genotype these loci in the large scale pig breeding programs.
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