| Summary: | 碩士 === 國立成功大學 === 醫學工程研究所碩博士班 === 95 === The neuroprotective properties of melatonin, a strong natural antioxidant and free radical scavenger which decreases oxidative lipid peroxidation and DNA hydroxylation, and prevents against energy depletion during ischemia, will be evaluated in Sprague-Dawley rats subjected to 1-hour transient middle cerebral artery (MCA) occlusion. A experiments with delayed treatment of melatonin will be employed to examine whether it is neuroprotective following a long period of recovery using a model of reversible MCA occlusion. Postmortem infarct volumes will be determined by quantitative image analysis of Nissle-stained brain sections and by immunohistochemical methods. In addition, postischemic electrophysiological recovery, neurogenesis, synaptic arborization and axonal sprouting will be evaluated. The possible mechanisms of action relevant to long-term neuroprotective efficacy by which melatonin may exhibit the neuroprotective effects will also be evaluated in the model. The data will not only extend our original findings to the ischemic-reperfusion injury but also look at prolonged outcomes, thus, may provide a potential outlook to treat patients against ischemic stroke.
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