Study the role of TLE2 in v-Src-mediated transformation

• Main Authors: Shang-hsien Chen, 陳尚賢
• Other Authors: Tzeng-horng Leu
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/11522555162784762073

碩士 === 國立成功大學 === 藥理學研究所 === 95 === Two isoforms, i.e. p97Eps8 and p68Eps8, were detected in many cultured cell lines by Eps8 antibody. Our previous studies indicated that p97Eps8 participates in v-Src-mediated tumorigenesis. To characterize Eps8-mediated signal transduction, we previously identified TLE2 (transducin-like enhancer of split 2) as one of its associated proteins by yeast-two hybrid screening. Further studies indicated that TLE2, as well as both Eps8 isoforms, was overexpressed in v-Src transformed IV5 cells. Interestingly, decreased expression of TLE2 was detected in Eps8-attenuated IV5 cells suggested its involvement in Eps8-mediated signal transduction. To study the role of TLE2 in cell proliferation and transformation, we transiently expressed tle2 into NIH3T3 cells or Eps8 overexpressing human kidney 293T cells. We found TLE2 overexpression could inhibit their anchorage-independent growth in soft agar. These findings suggested that TLE2 might be able to interact with Eps8 and inhibit the transforming ability of Eps8.