analysis of protein-protein and protein-DNA interactions during filamentous phage assembly

• Main Author: 徐晨圃
• Other Authors: 林苕吟
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/21416762171222116744

碩士 === 國立交通大學 === 生物科技系所 === 95 === Filamentous phages are small , highly evolved parasites that can reproduce and disseminate without killing their host . The genome of filamentous phage is single stranded circular DNA . The process by which filamentous phage are concomitantly assembled and secreted across the cell membranes is likely to involve a series of protein – protein and protein – DNA interactions .The previous findings that deletion of the filamentous phage packaging signal can be compensated for by mutations in g7p , g9p , and g1p , strongly suggest a role for these proteins in the initiation of assembly . In addition , thioredoxin is required for efficient filamentous phage production and may interact to g1p to drive an engine for filamentous phage assembly . This report tests the gel shift assay for examining previous proposal about protein- packaging signal interaction . We also investigate the possible interactions between these proteins by various assays. These results would show information on the structure and assembly process of filamentous phage .