Safety and evalution of hepatoprotective of Sang-Hwang

• Main Authors: Chung-Chi Wu, 吳忠錡
• Other Authors: Chin-Yin Tseng
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/59302163174626505787

碩士 === 國立嘉義大學 === 食品科學系研究所 === 95 === In traditional Chinese herbal medicine, Sang-Hwang is a kind of valuable medicinal mushroom. It is a group of perennial hard mushrooms which are parasite on mulberry and so forth. At overseas researches, it has been found many kinds of health care effects. Most of Sang-Hwang researches have been studied in Korea and Japan, and extremely few researches in Taiwan. Its origin is distributed from Japan, Korea to China, Australia, and North America. In chapter one, three kinds of Sang-Hwang strains, Phellinus igniarius、Phellinus linteus and Phellinus pini were used as material to analyze proximate composition, content of microelements and content of functional components. In antimutation test, Salmonella typhimurium TA98 and TA100 are experiment bacterial strains for testing Sang-Hwang water extracts by Ames test with direct mutation agent NQNO(-S9) and indirect mutation agent AFB1(+S9). The results showed that content of functional components, P. igniarius, P. linteus and P. pini polysaccharide content was 4.98 %, 2.54 % and 3.82 %, respectively. β- 1,3- glucan content of P. linteus was 5.29 mg/dw g, P. igniarius 4.34 mg/dw g, and P. pini 6.16 mg/dw g. In SOD-like activity, P. igniarius was 119.05 U/ml, P. linteus 69.23 U/ml and P. pini 59.00 U/ml. On the other hand, there were no toxicity and mutation reaction at 0.5~10 mg/plate for three kinds of Sang-Hwang. In chapter two, efficiency evaluation of P. pini protecting liver was in animal mode aimed at rat chemistry liver damage inducing with carbon tetrachloride. It was divided into normal group, carbon tetrachloride group (CCl4 liver damage group), Silymarin treatment group, high dosage group 100 mg/kg, middle dosage group 50 mg/kg, and low dosage group 10 mg/kg. The results showed that superoxide dismutase (SOD), glutamate oxaloacetate transaminase (GOT), glutamate pyruvate transaminas (GPT) all were downside. Through contents results of glutathione peroxidase (GPx) and glutathione reductase (GRd), the Silymarin treatment group and the experiment groups high, middle, low dosage had promoted effect, and significantly differenced from carbon tetrachloride group, obviously. From these whole knowable results, P. pini water extracts protecting liver effect were better in dosage 50 mg/kg than others. For this result, P. pini can combine with other protecting liver materials to develop as health food.