Spectroscopic study of nanodiamond-protein complexes and their interaction with bacteria and cells

• Main Authors: Chih-Yuan Cheng, 鄭智元
• Other Authors: Chia-Liang Cheng
• Format: Others
• Language: en_US
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/465t7u

碩士 === 國立東華大學 === 應用物理研究所 === 95 === Recently, researches on nano-scale materials have become important issues due to their perspectives applications in electronics, optics, and bio-medical technologies, etc. Particularly, nanodiamond (ND) is of interest because its excellent physical and chemical characteristics, such as high hardness, high electron resistivity, chemical stability, bio-compatibility, and so on. NDs attached with biomolecules on the surface can be used as bio-nano-probes in cell and tissue analysis. In this work, we developed bio-nano-probe on the base of NDs with detection in Raman signal and its intrinsic fluorescence. NDs used in this experiment are nanoparticles with average dimeter 5 nm and 100 nm. To demonstrate this, NDs were carboxylated and conjugated with proteins; using both physical adsorption and chemical linking (covalent binding). The cNDs’ surface properties and cND-protein interaction were analyzed using Fourier transform infrared spectroscopy (FTIR), UV/Vis spectroscopy, confocal fluorescence microscopy and Raman spectroscopy. We have investigated the interaction of cND-protein conjugates with bacteria and cells. The developed cND-protein complexes retained the functionality of protein, while NDs serve as probe for detection by using spectroscopic methods, auch as Raman spectroscopy and fluorescence microscopy.