| Summary: | 碩士 === 國防醫學院 === 公共衛生學研究所 === 95 === The military cooks had heavy burden of cooking work, they may expose to cook oil fumes in military kitchens. Cook oil fumes (COF) constituted a complex mixture of toxicants, including polycyclic aromatic hydrocarbons(PAHs) which may induce oxidative DNA damage. The purpose of this study was to study the relationships between COF exposure and the oxidative DNA damage.
We carried out a longitudinal study to explore the relationships. Exposure assessment based on a biomarker of PAHs exposure, urinary 1-hydroxypyrene（1-OHP）. Urinary 8-hydroxydeoxyguanosine (8-OHdG) was used as a biomarker of oxidative DNA damage. Study subjects were recruited from 3 military bases of the north, middle and southern part in Taiwan. The exposed group included 77 male military cooks exposed to COF. The control group consisted of 76 office soldiers not exposured to COF during the study period.
We distributed a self-administrated questionnaire to the subjects, which inquire about personal characteristrics, such as age, education, smoking habits, histories of disease, consumption of broiled, grilled and barbecued food,and use of personal protective equipment. Workers were asked to collect a pre-shift of the first weekday and a post-shift of the fifth workday urine samples.
The results showed that urinary 1-OHP of military cooks increased significantly (0.305→0.638 µg/g-creatinine) after 5 days exposure to COF. Multivariate GEE alalysis indicated military cooks has statistically significant elevated levels of 1-OHP than that of control group. On the other hand, urinary 8-OHdG of military cooks decreased (8.58→7.71 µg/g-creatinine) from pre-shift of the first day of workweek to post-shift of the last day of workweek. Multivariate GEE alalysis showed military cooks had no significant decreasing levels of 8-OHdG compared with control group. Spearman correlation analysises showed urinary 1-OHP was not correlated with urinary 8-OHdG. Multivariate GEE alalysis indicated that the change of urinary 1-OHP with time didn’t affected the change of urinary 8-OHdG.
Our results demonstrated that urinary 1-OHP could be a promising biomark of PAHs exposure to COF, whereas urinary 8-OHdG was not associated with 1-OHP to reflect oxidative DNA damage in military cooks.
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