Study on anti-hyperglycemic function of different plants

• Main Authors: Yu-Yi Chu, 朱育儀
• Other Authors: Pao-Chuan Hsieh
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/24643757965065840189

碩士 === 國立屏東科技大學 === 食品科學系所 === 95 === Diabetes mellitus (DM) is one of the metabolic disorders due to deficiency of insulin action. However, available drugs for DM have a number of limitations, such as adverse effects and high rates of secondary failure. Several studies have demonstrated many plants containing anti- hyperglycemic activity. The purpose of this study was to evaluate the anti-hyperglycemic activity of various plants. First, the inhibition of α-amylase activity was assayed in vitro. The results showed the water extracts from Psidium guajava leaves, Tinospora cordifolia stems and mixture of plant water extracts showed the highest inhibition of α-amylase activity and the IC50 was 3.24 mg/mL, 7.98 mg/mL and 3.14 mg/mL, respectively. In toxicity, mutagenicity of mixture of plant water extracts were investigated by Ames test. The results indicated that the mixture of plant water extracts (0.3125~5.0 mg/plate) couldn’t induce the toxicity and mutagenicity of Samlmonella typhimurium TA97 and TA100 with or without S9 mixture. In cytotoxicity, human hepatoma cell lines (HepG2) and murine embryonic liver cell line (BNL CL.2) which were treated with various concentrations of plant extracts, were used to investigate the cytotoxicity by MTT of assay（3- （ 4,5-dimethylthiazol-2-yl ）-2,5-diphenyltetrazolium bromide assay）. When the cells of BNL CL.2 were treated with 5~300 μg/ml of mixture of plant water extracts for 24, 48 and 72 hours, the survival percentages were significantly higher than control group (p＜0.05), but no significant effect on HepG2 were observed. In vivo, the Wistar rats were employed and introduced nicotinamide (230 mg/kg) and streptozotocin (75 mg/kg) for diabetic induces. After continuous feeding for 5 weeks of mixture of plant water extracts showed to reduce eating and increase body weight, and had a significant decrease in triglyceride, LDL, and creatinine concentration in plasma, and increase in HDL concentration in plasma. The level of blood glucose and was decreased on the third week of feeding and continuously the level of blood glucose stable to the end of experiment. In histopathological study, normal histograms of eyes, kidney, heart, pancreas, brain and liver tissue were observed in NC group, while various intensity of renal tube epithelium cell hydropic degeneration was found in other diabetic-induced groups (DC, 1DC, 5DC and 10DC). However, no histopathological changes were observed in other organs, such as brain, liver and eyes. Key words: Psidium guajava, Tinospora cordifolia, Ames test, anti-hyperglycemic