| Summary: | 碩士 === 國立屏東科技大學 === 獸醫學系所 === 95 === 100 Escherichia coli ( E. coli ) -suspected samples from 360 piglets that died from the E. coli suspected infections were cultured to isolate and detect these pathogens. 100 samples of 360 E. coli-suspected samples were identified to be Enterotoxigenic E. coli ( ETEC ) infections. The positive rate was 27 % ( 100 / 360 ). ETEC nucleic acid was extracted from pure culture colony and amplified by Multiplex polymerase chain reaction using the two specific primers that design for amplification of the heat-labile enterotoxin ( LT ) gene 360 bp or heat-stable enterotoxin ( ST ) gene ( STa 160 bp, STb 423 bp ), respectively. Results of the multiplex PCR analysis for detecting the LT1, STa and STb toxic genes of ETEC, the only possession of LT1 gene were 5 % ( 5 / 100 ), STa gene were 8 % ( 8 / 100 ), STb gene were 11 % ( 11 / 100 ), both possession of LT1 and STb genes were 29 % ( 29/100 ), and possession of the LT1, STa and STb genes were 47 % ( 47/100 ). A drug sensitivity test was performed to determine the sensitivity of isolated ETEC strains to 8 drugs. ETEC had resistant to most commercial drugs ( Ceftiofur, Cephalothin, Colistin, Enrofloxacin, Erythromycin, Gentamycin, Neomycin, Streptomycin, Sulfamethoxazole / Trimethoprim ), except for Ceftiofur, Colistin, Sulfamethoxazole / Trimethoprim. The findings of drugs resistance were indicated that neonatal and weaned piglets with colibacillosis caused by ETEC were usually difficult to be treated due to drug resistance. The results of the study may provide the information for clinical prevention and treatment of colibacillosis in piglets.
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