| Summary: | 碩士 === 國立清華大學 === 化學工程學系 === 95 === Recently, because of the general attention to the gene therapy, gene carriers become an important issue. The transfection efficiency of viral vectors is high, but there are some considerable problems such as the mutation. So far, the most well developed nonviral vectors are liposomes. Although liposomes are known as safer carriers, their transfection efficiency remains low. We used cationic liposomes composed of a cationic lipid DC-Chol and a neutral lipid DOPE. DC-Chol could form complexes with DNA through the electrostatic force, and DOPE helped the release of DNA.
Polyethylenimine (PEI) is a positively charged polymer. It induces the endosome to break and the lipoplexes could escape more easily. In this research, we co-placed the cationic liposome, PEI and DNA to form complexes, and then went on the transfection in CHO-K1 cells. The results showed that PEI can enhance 6-fold in the transfection efficiency; the cell viability remained higher than 60% with the appropriate amount of PEI; moreover, the existence of PEI could lower the serum sensitivity of the gene delivery system, and it was beneficial to improve the applicability on the in vivo experiments.
Therefore, the gene delivery system composed of PEI and cationic liposome was worthy of intensive research. We can use different cationic lipid to explore the general effects of PEI, or modify PEI to target the specific cells, and finally we can proceed with the in vivo experiment to develop a complete gene delivery system.
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