Novel silver tetrazole compounds development of hydrothermal synthesis for biological application discussion & SBA-15 as a drug carrier for aspirin release

• Main Authors: Chi-Hao Chung, 鐘啟豪
• Other Authors: Hsiu-Mei Lin
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/11632164260164937442

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 95 === Abstract The application of tetrazole compounds is extensively and can be a bioisosterer to replace a carboxylic acid . To take the idea of replacement of COOH functional group of CH3COOH and 4-Pyridine carboxylic acid (Isonicotinic acid) that have drug efficacy by tetrazole, compare compounds of silver tetrazole with silver carboxylic acid to realize the biological medicine differences of both. Make use of in situ tetrazole synthesis method to ligand with metal silver, and find a precursor of 4-pyridyl tetrazole to proceed nitriles(CN) and azide(N3) of [2+3]additional cyclization through in situ ligand hydrothermal method to make molecular self-assembled, yeild a Ag(4-pyridyl)tetrazole(Ag(4-ptz))(AgC6H4N5), a=7.3044(2), b=11.1025(2), c=8.5298(2), α =90°, β =97.6670(10)°, γ =90 °, crystal system of Monoclinic. At the biological medicine effect , the anti-bacterial experiment of the silver tetrazole (Ag(4-ptz)) compound are greatly part better inhibition than the Ag(Isonicotinic acid). Additionally, we do not obtain conclusions that the gram positive or negative bacterial has better inhibition advantage than one of them.The gram positive bacterial has advantage of negative electricity to reject with tetrazole, the gram negative bacterials has the lipopolysaccharide hydrophilic group that make advantage of tetrazole drug not easily getting into bacterial cell membrane. Moreover, the result of synergistic bacterial inhibition of silver and tetrazole has also been confirmed from experiment and literature of(Ag(tetrazole) ). Abstract Mesoporous material become a hot point of research because of its high potential on the large application in recent years. Because of its large surface area and large pore, high regular pattern and tunable Nanoporous radius, variable properties of surface chemistry, high hydrothermal stability, and easy preparation to synthesze, not time-consuming and the non-poisonous, high stability and the good biological compatibility of silicon material , they have already been regard as a material of the carrier to deliver medicine . Make use of aspirin loading on SBA-15 that synthesized by three kinds of condition and deliver it in imitate stomach liquid(PH=1.2) and intestines liquid(PH=6.8) of the aqueous solution to release, find as a result of releasing more and fast in pH 6.8 and the release sequence for:[80-24(pH 6.8)]>[100-24 (pH 6.8)]>[80-48(pH 6.8)]>[100-24(pH 1.2)]>[80-48(pH 1.2)]>[80-24(pH 1.2)], can observe a result that loading amount of drug and pore size both present positive relationship.Although the releasing of Aspirin/SBA-15 much faster than the Aspirin/MCM-41, leading to the more Aspirin content in stomach and not significantly reduce side effect as well as MCM-41.Compare with the intestines-dissolved tablet that need to wait for passing through stomach to collapse inside the intestines and have drug efficacy, the Aspirin/SBA-15 has already released in the stomach and acidic Aspirin in the acidity condition has been more absorbed from the stomach . Comparatively Aspirin/SBA-15 is good for impatient patient and such a nanoporous material of SBA-15 has a new additional application for drug release.