Manipulation of Wnt Signaling Through Retrovirus Mediated Gene Transfer

• Main Authors: Teng-Cheong Ha, 夏鼎昌
• Other Authors: Chung Leung Li
• Format: Others
• Language: en_US
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/16860549693915968843

碩士 === 國立臺灣海洋大學 === 生物科技研究所 === 95 === Abstract Retroviral mediated gene transfer is one of the widely used techniques for transferring genes into mammalian cells. There are cells which by its nature is hard to be transfected, stem cell is one of the kind. This includes mouse embryonic stem cells (ESCs) and haematopoietic stem cells (HSCs), two of the stem cell type that our lab is interested in. Especially when primary isolated HSCs do not have the advantages for long term in vitro culture for expansion. Thus a more efficient retrovirus gene transfer method is recommended for these cells. Here, we compare a total of four retroviral vectors in their capacity to generate high titer virus preparation and to examine the level of transgene expression among various fibroblasts, haematopoietic cells together with ES cells. We further tested our retrovirus gene delivery system with a constitutively activated beta-catenin and demonstrated the inhibition of adipogenesis in 3T3-L1 preadipocytes.