Study of Intracellular Signaling Transduction on Abalone Hemocyte Aggregation

• Main Authors: Chia-wei Ku, 顧家瑋
• Other Authors: Jiun-Hong Chen
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/82833227782141060012

碩士 === 國立臺灣大學 === 動物學研究研究所 === 95 === Hemocyte aggregation is the main function for wound repair and stop bleeding. In this study, hemocytes of abalone (Haliotis diversicolor) were used to investigate the mechanisms of intracellular signaling transduction on cell aggregation. Based on the results, galectin, one of galactose-sepecific and divalent cation-dependent binding proteins is involved to the aggregation of abalone hemocytes. About the signaling transduction, PMA(phorbol 12-myristate 13-acetate), a broad spectrum PKC(protein kinase C) activator、U73122, a PLC(phospholipase C) inhibitor, Gö6976, classical PKC inhibitor, significantly promoted the aggregation of abalone hemocytes; Contrarily, calcium ionophore A23187 and D-sphingosine, a broad spectrum PKC inhibitor inhibited the aggregation of abalone hemocytes. It is inferred that there are more than one type of PKCs involved in the aggregation of abalone hemocytes. During the period of aggregation of hemocytes, the total phosphorylation level of the hemocyte proteins is increased. In addition, one of STG(shrimp transglutaminase), STG1, secreted from shrimp(Marsupenaeus japonicus) hemocytes could influence the aggregation of abalone hemocytes.