Indentification of Molecular Markers Associated with Male Sterility System in Pepper (Capsicum annuum L.)

• Main Authors: Tzu-Chi Yang, 楊子奇
• Other Authors: Wen-Ju Yang
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/85993214585425389444

碩士 === 國立臺灣大學 === 園藝學研究所 === 95 === Abstract The identification molecular marker relevant to male sterility is very important in breeding program. Precise prediction of male sterility at seedling stage can certainly shorten the breeding protocol and schedule. According to the segregation ratio, the male sterility of the peppers form TSIPS (Taiwan Seed Improvement and Propagation Station) was controlled by a single gene. RAPD-BSA (random amplified polymorphic DNA - bulked segregant analysis) was used to identify potential markers; however, none of the 508 tested primers was able to identify either male sterile or fertile bulk including OPP13 and OPW19. Primer sets that designed according to the mitochondria apt6-2 gene and male sterility related protein ORF456 were able to identified the male sterile lines indicated that the tested TSIPS pepper lines contain male-sterile cytoplasm. The CRF-S primer set was reported to be specific to the nuclear fertility restorer gene, Rf, in hot pepper; such primers set was able to identified the male fertile progenies of the male sterile lines indicating that the material contained Rf gene. Therefore, the male sterility of the material belongs to genic-cytoplasmic male sterility system. In this study, other male sterile lines were collected from TSIPS and AVRDC (the World Vegetable Center) to test for the discriminate ability of the markers to male-sterility. The result of our study indicated that the primer sets for the male sterile cytoplasm were able to identify all the lines and the CRF-S primer could discriminate the Rf_ and rfrf genotype from all the collected lines except AVRDC-5032. Therefore, we concluded that the primer sets we used could be potentially applied in pepper breeding program.