Development of a Closed Loop High-Throughput Microfluidic PCR Chip and System

• Main Authors: Chiu-Chi Lai, 賴秋吉
• Other Authors: Yuan-Nan Chu
• Format: Others
• Language: zh-TW
• Published: 2006
• Online Access: http://ndltd.ncl.edu.tw/handle/13412075722599016560

碩士 === 國立臺灣大學 === 生物產業機電工程學研究所 === 95 === This research has developed a novel disposable closed-loop PCR chip which allows non-restricted number of thermal cycles on a small footprint. The fluid in the microchannel of the PCR chip is driven by mechanically compressing the microchannel using wheels pressing on the channel in only one direction to precisely control the flow to loop through three temperature zones. An automatic driving mechanism is also developed to achieve high throughput by driving multiple chips at the same time on a single thermal platform. The PCR chip is made by bonding a PDMS top layer to a glass substrate. The PDMS top layer is casted on a PMMA mold. The channel pattern on the mold is machined on a CNC machine then furbished by hand. The PDMS cover is then treated by O2 plasma and bond to the glass substrate. The microchannel is 300 μm wide, 100 μm high, with a total loop length of 282.24 mm to contain about 13.1 μl of fluid when full. Fluid to be processed is simply dropped onto a well of the I/O port and then taken into the channel loop automatically by the driving mechanism. When the PCR process completes, the fluid is again released through the same port. Simultaneous PCR amplification results have been successfully demonstrated on two chips. The reported PCR chip is the first flow-type PCR chip that could fully prevent cross contamination within the microchannel.