Surface modification of gelatin nanoparticles with polyethylenimine as a gene vector

碩士 === 臺灣大學 === 醫學工程學研究所 === 95 === In gene therapy, developing non-toxic and efficient gene delivery system is vital. Although there has been numerous studies regarding viral gene vectors, safety concern limits the application. For this reason, recent studies have shown that non-viral gene vectors...

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Main Authors: Meng-Chiao Wu, 吳孟樵
Other Authors: 黃義侑
Format: Others
Language:zh-TW
Published: 2007
Online Access:http://ndltd.ncl.edu.tw/handle/19029385785406684079
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spelling ndltd-TW-095NTU055300232015-10-13T13:55:54Z http://ndltd.ncl.edu.tw/handle/19029385785406684079 Surface modification of gelatin nanoparticles with polyethylenimine as a gene vector 利用聚乙二烯胺修飾作為基因載體的明膠奈米微粒 Meng-Chiao Wu 吳孟樵 碩士 臺灣大學 醫學工程學研究所 95 In gene therapy, developing non-toxic and efficient gene delivery system is vital. Although there has been numerous studies regarding viral gene vectors, safety concern limits the application. For this reason, recent studies have shown that non-viral gene vectors, especially polymer nanoparticles, play an important role in gene therapy. Polymer nanoparticles offer several significant advantages, including biosafety, easier modification, cost-effectiveness and so on; polyethylenimine is particularly attractive for non-viral gene therapy. The high affinity with DNA of PEI is due to its large amount of positive net charges from nitrogen atoms. High cationic surface charge also gives PEI an excellent buffer capacity in acidic environment. However, high transfection efficiency of PEI (25kDa), along with its cytotoxicity, strongly depends on its molecular weight. To maintain the transfection efficiency and minimize cytotoxicity, many studies performed cross-linked low molecular weight PEI. In this study, gelatin, a biopolymer without cytotoxicity, was made to nanoparticle. Then PEI was cross-linked into the surface of nanoparticles. Because of high positive zeta potential (42.47 mV) and buffering effect, GA-PEI 1.8k can form compact and stable complex with DNA. The results showed that GA-PEI 1.8k NPs with N/P ratio 30 had excellent transfection efficiency (2.12×104 RLU/μg Protein), which is about 20% of ExGen 500(1.17×105 RLU/μg Protein)and comparable to Lipofectamine 2000 (3.12×104 RLU/μg Protein). The cell viability of GA-PEI 1.8k (86.4%) is higher than ExGen 500 (67.7%) and Lipofectamine 2000 (33.4%). We successfully cross-linked PEI into the surface of gelatin nanoparticle. As a gene vector, GA-PEI 1.8k NPs had high trnasfection efficiency and low cell toxicity, and it can be a potential gene delivery system used in gene therapy. 黃義侑 2007 學位論文 ; thesis 50 zh-TW
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description 碩士 === 臺灣大學 === 醫學工程學研究所 === 95 === In gene therapy, developing non-toxic and efficient gene delivery system is vital. Although there has been numerous studies regarding viral gene vectors, safety concern limits the application. For this reason, recent studies have shown that non-viral gene vectors, especially polymer nanoparticles, play an important role in gene therapy. Polymer nanoparticles offer several significant advantages, including biosafety, easier modification, cost-effectiveness and so on; polyethylenimine is particularly attractive for non-viral gene therapy. The high affinity with DNA of PEI is due to its large amount of positive net charges from nitrogen atoms. High cationic surface charge also gives PEI an excellent buffer capacity in acidic environment. However, high transfection efficiency of PEI (25kDa), along with its cytotoxicity, strongly depends on its molecular weight. To maintain the transfection efficiency and minimize cytotoxicity, many studies performed cross-linked low molecular weight PEI. In this study, gelatin, a biopolymer without cytotoxicity, was made to nanoparticle. Then PEI was cross-linked into the surface of nanoparticles. Because of high positive zeta potential (42.47 mV) and buffering effect, GA-PEI 1.8k can form compact and stable complex with DNA. The results showed that GA-PEI 1.8k NPs with N/P ratio 30 had excellent transfection efficiency (2.12×104 RLU/μg Protein), which is about 20% of ExGen 500(1.17×105 RLU/μg Protein)and comparable to Lipofectamine 2000 (3.12×104 RLU/μg Protein). The cell viability of GA-PEI 1.8k (86.4%) is higher than ExGen 500 (67.7%) and Lipofectamine 2000 (33.4%). We successfully cross-linked PEI into the surface of gelatin nanoparticle. As a gene vector, GA-PEI 1.8k NPs had high trnasfection efficiency and low cell toxicity, and it can be a potential gene delivery system used in gene therapy.
author2 黃義侑
author_facet 黃義侑
Meng-Chiao Wu
吳孟樵
author Meng-Chiao Wu
吳孟樵
spellingShingle Meng-Chiao Wu
吳孟樵
Surface modification of gelatin nanoparticles with polyethylenimine as a gene vector
author_sort Meng-Chiao Wu
title Surface modification of gelatin nanoparticles with polyethylenimine as a gene vector
title_short Surface modification of gelatin nanoparticles with polyethylenimine as a gene vector
title_full Surface modification of gelatin nanoparticles with polyethylenimine as a gene vector
title_fullStr Surface modification of gelatin nanoparticles with polyethylenimine as a gene vector
title_full_unstemmed Surface modification of gelatin nanoparticles with polyethylenimine as a gene vector
title_sort surface modification of gelatin nanoparticles with polyethylenimine as a gene vector
publishDate 2007
url http://ndltd.ncl.edu.tw/handle/19029385785406684079
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