Evaluation of Epitheliotrophic Capacity of Four Different Blood Derivatives on Bovine Corneal Epithelial cells

• Main Authors: Shao-Hui Cheng, 鄭劭蕙
• Other Authors: Chung-Tien Lin
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/99659962085711949174

碩士 === 國立臺灣大學 === 獸醫學研究所 === 95 === PURPOSE： Human serum eye drops have been successfully used in treatment of severe ocular surface disorders and enhancement of corneal wound healing. Umbilical cord serum is effective in treatment of dry eye and persistent corneal epithelial defects. Fresh frozen plasma has not yet been tested for use as eye drops in patients, although it is easily available as quality-controlled products from blood banks. Fresh frozen plasma or umbilical cord serum could be used for substituting human serum when the source of human serum lacks. The use of fetal bovine serum (FBS) in the culture medium has been a major concern worldwide because of bovine spongiform encephalitis (BSE). The use of other human source blood-derived products to replace FBS in the culture process would help. We compared these four blood products by investigating the epitheliotrophic capacity in an in vitro model of bovine epithelial cell monolayer. MATERIALS AND METHODS：Primary cultured bovine corneal epithelial cells were used to investigate wound healing, cell proliferation and migration by means of scratch corneal wound evaluation, MTS assay and Boyden chamber migration assay in response to human serum, umbilical cord serum, fresh frozen plasma and FBS. The concentrations of EGF, TGF-β1, and fibronectin in human serum, umbilical cord serum and fresh frozen plasma were evaluated by ELISA kits. RESULTS： The effect of promoting corneal epithelial wound healing of FBS was better than three other human sourced blood products. Cell proliferation and migration were best enhanced by FBS, followed by umbilical cord serum and human serum, and were worst in fresh frozen plasma. Growth factor (EGF and TGF-β1) concentrations were significantly higher in umbilical cord serum than in human serum and were lowest in frozen plasma. The concentration of fibronectin in frozen plasma was significantly higher than in human serum and was lowest in umbilical cord serum. CONCLUSIONS： Umbilical cord serum and fresh frozen plasma may possess potential for substitution of human serum to treatment ocular surface disease and enhance corneal wound healing. Although the enhancement of epitheliotrophic capacity in human-sourced blood products was not as excellent as FBS, these three blood products could also promote proliferation and migration of corneal epithelial cells. These products could be useful materials in cultivating cells for clinical use.