The circadian rhythm-related gene expression of liver and kidney in a metabolic syndrome-like mice model

• Main Authors: Ming-Chen Hsieh, 謝明蓁
• Other Authors: Kun-Ruey, Shieh
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/63127141814520328642

碩士 === 慈濟大學 === 整合生理暨臨床科學研究所 === 95 === Physiological and behavioral circadian rhythmicities in mammals are dominated by the central circadian pacemaker, the hypothalamic suprachisamatic nucleus. These circadian rhythms are generated at an intra-cellular level by circadian oscillators, which are composed of transcriptional/translational feedback loops involving a set of circadian rhythm-related genes, such as Period (Per) 1-3, Cryptochome (Cry) 1-2, Clock, Bmal1, Dbp, E4bp4, and CK1ε. Interestingly, these circadian rhythm- related genes exist in not only the hypothalamic suprachiasmatic nucleus but also various peripheral tissues. Whether the impairments of the circadian rhythm-related genes in peripheral tissues are involved in the development of metabolic abnormality and contribute to a wide range of physiologic disorders are the main focuses of this study. To address this issue, we used a high fat diet to induce the male C57BL/6J mice as the obese, hyperleptinemic, hyperinsulinemic and hypertriglyceridemic models. Using the real-time quantitative PCR (RT-qPCR) methods, we found the rhythmic expression of circadian rhythm-related genes including: Clock, Bmal1, Per1-3, and Cry1-2, clock-controlled downstream genes: Dbp, E4bp4 and negative elements kinases: CK1ε, were altered in liver and kidney. These results exhibit that high fat diet-induced obesity alters the circadian related genes system in mouse peripheral tissues. According to our present data and previous papers, obesity and metabolic syndrome are highly correlated to the changes of circadian-rhythm related gene expression.