Characterization of ftsX gene of Helicobacter pylori

• Main Authors: Wen-chie Huang, 黃文杰
• Other Authors: Nien-tsung Lin
• Format: Others
• Language: zh-TW
• Online Access: http://ndltd.ncl.edu.tw/handle/49980879340099838525

碩士 === 慈濟大學 === 微免暨分子醫學研究所 === 95 === FtsE and FtsX belong to the ABC（ATP-binding cassette） transporter superfamily of and appear to be widely conserved among bacteria. So far, the role of the ftsE and ftsX genes are controversial. They were initially reported to be involved in cell division in Escherichia coli, and were subsequently proposed to be components of the septalsome, translocating periplasmic components of the cell division apparatus. In this study, we would like to characterize the ftsE and ftsX function in Helicobacter pylori. We constructed ftsE and ftsX mutant of H. pylori NCTC 11637 by homologous recombination with chloramphenicol-resistant marker. The growth curve of ftsE and ftsX mutant showed the distinct from wild type, and disrupted ftsE and ftsX gene would affect the cell division then resulted in the filamentation phenotype. Also, the chromosome distribution exhibited unseparated DNA of ftsX mutant, caused part of bacterium absence of DNA. On the other hand, the tolerance of high salt medium in ftsX mutant was superior to wild type. In addition, we constructed ftsX complementary strain using flaA promoter to express ftsX, but didn’t restore the function.