Determination of cisplatin with platinum microelectrode

• Main Authors: Chi-Hao Chen, 陳繼浩
• Other Authors: Meng-Shan Lin
• Format: Others
• Language: zh-TW
• Published: 2007
• Online Access: http://ndltd.ncl.edu.tw/handle/70986661239899473221

碩士 === 淡江大學 === 化學學系碩士班 === 95 === A novel method for the fast detection of cisplatin by platinum microelectrode has been developed. The typical electrochemical behavior of cisplatin in different parameters including pH, concentration of chloride ion, material of working electrode and its surface condition has been investingated by cyclic voltammetry. Subsequently, cisplatin has measured by differential pulse voltammetry (DPV) using a microelectrode with effective radius of 13.6μm. This method involves a potential pretreated process to renew the activity of electrode surface. In contrast to a series of measurements without the pretreatment process, the relative standard deviation (n = 8) could be reducing from 44.3% to 1.2% compared with original process. After careful optimization, the analytical performance of current microelectrode was evaluation in the buffer solution of 0.05M, phosphate buffer pH7.4 containing 0.103M sodium chloride. The linear range of cisplatin is between 5μM to 200μM (R =0.999) with sensitivity of 3.383×10-6A/M. The estimated detection limit of this scheme is about 460 nM (S/N=3) and the relative standard deviation of twenty respective detection measurement of 20 μM cisplatin is 1.78%.