| Summary: | 碩士 === 國立雲林科技大學 === 電子與資訊工程研究所 === 95 === The theorem of this research applies the localized surface plasmon resonance of gold nanoparticles. The absorbance spectrum varied with different refraction indices and dielectric constants of the environment.Fabricated LSPR biosensors have a high sensitivity, high resolvability and being capable of quantitatively analyzing nano-scale bio-molecules in real-time and labeling-free. We use 5nm gold nanoparticles to make self-assembled monolayer(SAM) and multilayers nano-thinfilms on MPTMS modified glass substrates. Then those films were used to detect silver ion, to analyze the immunoassay of antigen and antibody by UV-VIS spectrophotometers, and to test sucrose solution with different refractive indices.The results show that the signal will vary linearly under different refractive index and the different gold nanoparticle layers affect the linearity. The gold nanoparticles, modified by the organic functional group, show execellent selectivity, and effectively binding with silver ions. Besides, the absorbance and peak wavelength of these self-assembled films show obvious red-shifted. For detecting silver ion solutions, the detection limit can reach to 0.5×10-3 M. The sensitivity results of immunoassay for Mouse antigen, antibody and BSA can attain down to 0.5ng/ml. Self-assembled nano-thinfilms, either monolayer or multilayers, show promising results for LSPR biosensors.
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