Transduction of Notch family proteins by using magnetic nanoparticles and its applications

• Main Authors: Ya-ting chang, 張雅婷
• Other Authors: Wen-chien Lee
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/89857536187720434590

碩士 === 國立中正大學 === 化學工程所 === 96 === Notch signaling has been shown to play an important role in maintaining the progenic cell state and decision of cell-fates like proliferation and differentiation. Previous studies revealed that in Notch signaling pathway, Notch-1 intracellular domain (N1IC) could continue activating downstream E (spl) / HES transcription factors via the combination with the function molecule of CSL to regulate cell differentiation and proliferation; or activating Mash-1 gene to suppress the production of some proteins which associate with cell differentiation, and prevent the cells from differentiateon. This study consists of two major parts. First, we cultured granulocyte colony-stimulating factor（G-CSF） mobilized peripheral blood precursor cells in order to yield mesenchymal stem cells. When we cultured a few days, we could observe some fibroblast-like and small round cells. The number of cells could increase, but very slowly. After passages, the cell morphology changed, and the cells stopped to proliferate. We conjecture that the cells might become mature cells. Second, we used a high efficient method for the transduction of MSCs and HeLa cells with protein of N1IC and investigated the role of N1IC in the in vitro proliferation and suppression of cell apoptosis. The results suggest that the transduction of N1IC could enhance the proliferation of MSCs and maintain MSCs in the progenitor state. The transduction of N1IC could also suppress HeLa cells apoptosis which was induced by diosgenin.