（1）Cystamine conjugated Au nanospheres and nanorods as DNA delivery systems（2）Effective DNA delivery with novel Biotin-ℓPEI and study of its cellular uptake pathway leading to transfection

• Main Authors: Chih-cheng Yu, 余志誠
• Other Authors: Jong-yuh Cherng
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/57955236273089251233

碩士 === 國立中正大學 === 化學所 === 96 === Abstract Recently,Gene delivery systems have been studied intensively applied broadly, but their clinical applications are still to be tested. Scientists are working hard to develop an efficient and safe delivery system for gene therapy. At the present time, major gene delivery systems employ either viral or nonviral vectors. However, viral vectors have serious problems in their safe and production concerns. Although nonviral vectors bear lower transfection efficiency as viral vectors, scientists are focusing on improving nonviral vectors to achieve on optimal efficiency. The aim of this study is to synthesize a new cationic material for gene delivery. Moreover, to understand the passage of DNA/Cationic polymer complexes via cullular uptake,inhibitors of endocytosis were used. Our findings could shed a light on the development of optimal non-viral gene delivery systems. The cellular uptake of Biotin-ℓPEI polyplex leading to effective transfection is thought to be dependent on one exclusive pathway（clathrin-mediated）.