| Summary: | 碩士 === 國立中正大學 === 地震研究所暨應用地球物理研究所 === 96 === In Taiwan, mud volcano is a spectacular geological structure and natural landscape. Its geographic distribution, outlook description and gas composition have been investigated by many researchers. However, few studies have been performed for the microorganism population and the application of potential energy. This research consists of those contents. One was to measure potential energy using single-chamber microbial fuel cells (MFC). In this case, the mud and the microorganism from Wu-Shan-Ding mud volcano were used as medium and biocatalysis for electricity generation. The results showed that power generated was 1.8μW, which corresponded to the current of 0.016mA. The power data compared with other MFC were lower. It would be because the number of the bacteria in the mud volcano was lower than the other soil. In addition, the polymeric material (i.e. biofilm) has not been found in anode surface. Nevertheless, the current and powder output were stable and sustainable for long periods using different resistances.The other one was to investigate the microbial distribution and diversity using polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE). The 16s rDNA sequences from PCR-DGGE bands were used to identify bacterial strain. The gas composition of Wu-Shan-Ding mud volcano was 99% of methane. However, non-methane medium was used to exclude the methanogens. Following the comparison with NCBI gene bank data, the possible species found in the mud volcano were Uncultured bacterium-1 (1-2), Uncultured bacterium-2 (1-5), Uncultured bacterium -3 (4-1), Uncultured bacterium -4(4-4), Uncultured bacterium -5 (4-6), Unidentified bacterium (1-2), Clostridium thiosulfatireducens (1-5), Frigovirgula patagoniensis(4-4), Pseudomonas aeruginosa (4-6). The 16s rDNA gene sequences of DGGE showed less than 97% of similarity to known strains in the database of NCBI. This implied more uncultureable strains have been found in this study. Clostridium was related with sulfide formation, which would be linked with sulfide composition in Wu-Shan-Ding mud volcano. In addition, Clostridium and Pseudomonas were applied to MFC in literature review. This research explored the understanding of microorganism diversity and the application of the mud volcano. Some suggestions were made for the possible use of mud volcano as a possible material for MFC. This work has outlined a method by which electricity generation can be performed from the mud volcano.
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