| Summary: | 碩士 === 國立中正大學 === 機械工程所 === 96 === Cell is the smallest biologically functional unit in lives. To understand the information of the cell, the present study investigated dielectrophoresis (DEP) chips containing insulating microstructures to create non-uniform electric fields for inducing DEP of cells in microsystems. Using various frequencies, the cells were successfully trapped at the constriction. In theoretical analysis and simulation, CFD-ACETM was used to simulate the field gradient which is proportional to the DEP force and to understand the influence of different geometrical parameters, thus, the optimal parameters were selected. By using the MEMS technology, the electrodes were fabricated on the glass substrate and the microchannel made of polydimethylsiloxane (PDMS) was bonded on the glass by oxygen plasma bonding. The experimental results showed that the cells survive well after resuspended in the sucrose solution of 8.62% in weight percentage with low electric conductivity. Upon different frequencies, the dielectrophoretic behavior of 15
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