Preparation and characterization of high-water-content hyaluronic acid hydrogel

• Main Authors: Jhih Ren Li, 李智仁
• Other Authors: S. W. Tsai
• Format: Others
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/26402969698027606690

碩士 === 長庚大學 === 生化與生醫工程研究所 === 96 === The physiological properties of hyaluronic acid (HA) makes it a promising material for a variety of application. However, in the dissolved state, pure HA is hinder by the short residence time and lack of mechanical properties. To improve the degradation rate, poor mechanical properties and clearance, chemical modifications or cross-linked to form hydrogel or matrix are required when it is designed to be applied. As reported in most of literatures, HA was chemically crosslinked with EDC or GA to produce lower water content matrix (around 60%) that resulted in loss the intrinsic high hydration character of HA. Therefore, the aim of this study is to develop a new crosslinked process which is not only maintain the high water content ability of HA, but also could enhance the biostability and mechanical property. Our results shown water content of HA hydrogel is higher than 95% and remarkably lower mass loss compared with unreacted HA by thermogravimetrec measurement. Moreover, in vitro cytotoxicity test showed that there was not any relative toxic effect of the HA hydrogel. A body weight of 200g guinea pig model was used to investigated the tissue effect and biodegradation rate of HA hydrogel. In this model, HA hydrogel crosslinked with polyethyl glycol diglycidyl ether (PEGDE) performed similar effect compared with commercial product after 6 months of implantation.