| Summary: | 碩士 === 嘉南藥理科技大學 === 藥物科技研究所 === 96 === Abstract
Cordyceps sinensis (CS) is an expensive tonic Chinese herbal medicine. However, people look for a similar pharmacological effects substitute of CS when many counterfeits and mimics of CS always found in markets, while Cordyceps militaris (CM) is a popularly using and cheaper natural of CS. The purpose of this study was evaluated the chemopreventive effects of cultured fruiting body of CM in mitomycin C-induced toxicity. We had started to evaluate the acute toxicity of cultured fruiting body of CM at SD rats and ICR mice.
A single maximum dose (2000 mg/kg) of CM were given by oral gavage (o.p.), we had measured the body weights of all animals after 7 and 14 days. Then we planed to evaluate the genotoxicity of cultured fruiting body of CM by the micronucleus test. We had sampled bone marrow of rats at 48 hours with 2000 mg/kg CM (o.p.), and collected the orbital peripheral blood of mice at 24, 48 and 72 hours with same treatment. The micronucleus test is an evaluated genotoxicity method by counting how many reticulocytes contained micronuclei (MN) in 1000 reticulocytes. And mitomycin C (MMC) is a famous anti-tumor cytotoxic drug. In our chemopreventive evaluations of cultured fruiting body of CM, we had designed to pre-treat six weeks age ICR mice with 125, 250, 500 and 1000 mg/kg CM (o.p.) for seven days, then given 0.5 mg/kg or 1 mg/kg MMC by intraperitoneal (i.p.) at the 7th day, than collected orbital peripheral blood of mice to counted the frequency of MN after 24, 48 and 72 hours treated mitomycin C.
The cultured fruiting body of CM were confirmed no acute toxicity or genotoxicity in this studies. In the chemopreventive evaluation, we found that CM decreased the frequency of MN at 24 hours (1000 mg/kg CM) and 72 hours (500, 1000 mg/kg) (p<0.05). Cordycepin, the well known active ingredient of CM, also reduce the mitomycin C-induced MN in a dose-dependent manner. In this study, our results suggest that CM can prevent mitomycin C-induced genotoxicity, and the active ingredient cordycepin may be involved in this chemopreventive effect.
In the COMET assay, which can detect the DNA damage, 1000 mg/kg CM also prevent 0.5 mg/kg MMC-induced COMET tail length. In LDH test, 1000 mg/kg CM can significant prevent 0.5 mg/kg MMC-induced cytotoxicity (p<0.001). From the above results, we had suggested that the cultured fruiting body of CM can against MMC-induced toxicity.
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