| Summary: | 碩士 === 中山醫學大學 === 口腔醫學研究所 === 96 === To search effectors of ZAK and to study the ZAK signaling cascade, our laboratory used a yeast two-hybrid system to isolate ZAK associated proteins from a human heart cDNA library. One of the isolated cDNAs encoded Rho GDP dissociation inhibitor beta (RhoGDIβ). RhoGDIβ, also known as Ly-GDI or D4-GDI, which belongs to a family of Rho GDP dissociation inhibitors that includes RhoGDIα, RhoGDIβ and RhoGDIγ is thought to regulate many biological activities in cells and also regulate the activity and localization of Rho family proteins. RhoGDIβ is almost exclusively expressed in hematopoietic lineages. In the lab, we discovered that RhoGDIβ induced hypertrophic growth of a cultured rat cardiac cell line, H9c2. In order to study the mechanisms of RhoGDIβ to regulate hypertrophic growth, we used a yeast two-hybrid system to determine RhoGDIβ associated protein from a human heart cDNA library. Only four yeast colonies were growing in selection plates and we isolated these cDNAs. We then identified two possible RhoGDIβ associated proteins, RhoA and MYBPC3, after sequencing these cDANs. We set to determine the association of these proteins with RhoGDIβ in vivo in this study.
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