SNWE and SNPE -mediated human hepatocellular carcinoma cells apoptosis and mechanism research

• Main Authors: Pei-Jun, 鍾佩君
• Other Authors: Chau-Jong Wang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/57071336412678353666

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 96 === Solanum nigrum L. is worldwide weeds of arable land, gardens. It is believed to have anti-oxidative, anti-inflammatory, hepatoprotection and anti-tumor effects. In China, it has been used in traditional folk medicine to treat different cancers. In this study, we demonstrated that the water extract of Solanum nigrum L. (SNWE) expressed cytotoxic effects to many cancer cell lines. Among them, human hepatocellular carcinoma cells (HepG2) were the most susceptible to SNWE. We also isolated the polyphenolic compounds (SNPE) form SNWE and then used MTT assay to evaluate the cytotoxicity on the HepG2 cells. The results show that HepG2 cells were more susceptible to SNPE than SNWE. Next, we observed the effects of SNWE and SNPE in inducing apoptosis in HepG2 cells by measuring the nuclear condensation, DNA fragmentation and increased the subG1 phase ratio in cell cycle. This effect of SNWE and SNPE in HepG2 cells might be mediated via increase the expression of Fas and cleaved-caspase 8, 3 protein. Furthermore, we treatment low dose of SNWE and SNPE to HepG2 cells and resulted in significant cell cycle arrest in G2/M phases. It might be due to decrease CDC25C protein expression. Besides in vitro effects, the antitumor efficacy of SNWE and SNPE were also confirmed in human hepatocellular carcinoma xenografts in nude mice. Thus, our data indicate that SNWE and SNPE could play an active role in mediating the cell cycle arrest and apoptosis of human hepatocellular carcinoma cells and might be potential drugs for antitumor therapy.