Inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation
碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 96 === High level of blood glucose could produce reactive oxygen species (ROS) to cause retinopathy. Our previous study demonstrated that Hibiscus sabdariffa aqueous extracts (HSE) and Hibiscus sabdariffa polyphenols extracts (HPE) could decelerate retinopathy in str...
Main Authors: | , |
---|---|
Other Authors: | |
Format: | Others |
Language: | zh-TW |
Published: |
2008
|
Online Access: | http://ndltd.ncl.edu.tw/handle/67405674735402344418 |
id |
ndltd-TW-096CSMU5107033 |
---|---|
record_format |
oai_dc |
spelling |
ndltd-TW-096CSMU51070332015-10-28T04:07:07Z http://ndltd.ncl.edu.tw/handle/67405674735402344418 Inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation 植物多酚單品對高濃度葡萄糖誘導之人類視網膜色素上皮細胞增生之抑制作用 Tzu-Ya 吳慈雅 碩士 中山醫學大學 生化暨生物科技研究所 96 High level of blood glucose could produce reactive oxygen species (ROS) to cause retinopathy. Our previous study demonstrated that Hibiscus sabdariffa aqueous extracts (HSE) and Hibiscus sabdariffa polyphenols extracts (HPE) could decelerate retinopathy in streptozotocin (STZ)-induced diabetic rats. In this study, we further examined the effect of protocatechuic acid, catechin, ferulic acid, gallic acid, quercetin and epicatechin existing in HSE in high glucose treating human retinal pigment epithelial (RPE) cells. We found that after treatment of high glucose (25 mM) for at least 6 days, RPE cells showed a significant increase in a dose-dependent manner. As accompanied with treatment of 10μM concentration of protocatechuic acid, quercetin, ferulic acid, catechin, gallic acid and epicatechin the increased cell could be reduced up to 51.78%, 37.4 %, 30.27 %, 30.07 %, 22.93%, 32.47%, respectively. We further examined the expressions of PKC-α, PKC-δ, PKC-λ. But, there were no significant changes in these proteins. We examined that after treatment of high glucose (25 mM) for 48 hrs, RPE cells showed the activity of MMP-2 significant increase.As accompanied with treatment of10μM concentration of protocatechuic acid, quercetin and ferulic acid the increased MMP-2 activity could be reduced to 25.9%, 14.8%, 5.9%, respectively. In conclusion, protocatechuic acid、quercetin、ferulic acid、catechin、gallic acid and epicatechin could reduce high glucose induced RPE cell proliferation, but the mechanisms should be further clarified. 李彗禎 2008 學位論文 ; thesis 66 zh-TW |
collection |
NDLTD |
language |
zh-TW |
format |
Others
|
sources |
NDLTD |
description |
碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 96 === High level of blood glucose could produce reactive oxygen species (ROS) to cause retinopathy. Our previous study demonstrated that Hibiscus sabdariffa aqueous extracts (HSE) and Hibiscus sabdariffa polyphenols extracts (HPE) could decelerate retinopathy in streptozotocin (STZ)-induced diabetic rats. In this study, we further examined the effect of protocatechuic acid, catechin, ferulic acid, gallic acid, quercetin and epicatechin existing in HSE in high glucose treating human retinal pigment epithelial (RPE) cells. We found that after treatment of high glucose (25 mM) for at least 6 days, RPE cells showed a significant increase in a dose-dependent manner. As accompanied with treatment of 10μM concentration of protocatechuic acid, quercetin, ferulic acid, catechin, gallic acid and epicatechin the increased cell could be reduced up to 51.78%, 37.4 %, 30.27 %, 30.07 %, 22.93%, 32.47%, respectively. We further examined the expressions of PKC-α, PKC-δ, PKC-λ. But, there were no significant changes in these proteins. We examined that after treatment of high glucose (25 mM) for 48 hrs, RPE cells showed the activity of MMP-2 significant increase.As accompanied with treatment of10μM concentration of protocatechuic acid, quercetin and ferulic acid the increased MMP-2 activity could be reduced to 25.9%, 14.8%, 5.9%, respectively.
In conclusion, protocatechuic acid、quercetin、ferulic acid、catechin、gallic acid and epicatechin could reduce high glucose induced RPE cell proliferation, but the mechanisms should be further clarified.
|
author2 |
李彗禎 |
author_facet |
李彗禎 Tzu-Ya 吳慈雅 |
author |
Tzu-Ya 吳慈雅 |
spellingShingle |
Tzu-Ya 吳慈雅 Inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation |
author_sort |
Tzu-Ya |
title |
Inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation |
title_short |
Inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation |
title_full |
Inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation |
title_fullStr |
Inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation |
title_full_unstemmed |
Inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation |
title_sort |
inhibitory effect of the pure compounds existing in plant polyphenols on high glucose induced human retinal pigment epithelial cell proliferation |
publishDate |
2008 |
url |
http://ndltd.ncl.edu.tw/handle/67405674735402344418 |
work_keys_str_mv |
AT tzuya inhibitoryeffectofthepurecompoundsexistinginplantpolyphenolsonhighglucoseinducedhumanretinalpigmentepithelialcellproliferation AT wúcíyǎ inhibitoryeffectofthepurecompoundsexistinginplantpolyphenolsonhighglucoseinducedhumanretinalpigmentepithelialcellproliferation AT tzuya zhíwùduōfēndānpǐnduìgāonóngdùpútáotángyòudǎozhīrénlèishìwǎngmósèsùshàngpíxìbāozēngshēngzhīyìzhìzuòyòng AT wúcíyǎ zhíwùduōfēndānpǐnduìgāonóngdùpútáotángyòudǎozhīrénlèishìwǎngmósèsùshàngpíxìbāozēngshēngzhīyìzhìzuòyòng |
_version_ |
1718112358538149888 |