| Summary: | 碩士 === 大葉大學 === 生物產業科技學系 === 96 === The goals of this research is to analyze and extract active components of polysaccharides、adenosine and triterpenoids from timber cultivated Antrodia camphorata as well as to compare the amount of polysaccharides、adenosine and triterpenoids in various mycelia derived from solid culture media. The results indicated that mycelia grown on different media, showed different levels of active components. The mycelia from medium 6 have the highest amount of polysaccharides(25.93 g/kg) and adenosine (745.15 mg/kg)；The mycelia from medium 3 have the highest amount of triterpenoids.
In comparison of different extraction techniques by using 20% ethanol as the solvent, the yield of polysaccharides using microwave-assisted extraction (MAE) for 9 minutes were equivalent to the 82% yield using heating reflux extraction (HRE) for 3 hours. As 20% ethanol was used for the solvent, the yield of adenosine using microwave-assisted extraction (MAE) for 7 minutes were superior to those using ultrasonic-assisted extraction (UAE) for 40 minutes and heating reflux extraction (HRE) for 3 hours. When 70% ethanol was used as the solvent, the yield of triterpenoids using microwave-assisted extraction (MAE) for 9 minutes were equivalent to the 91% yield for that of ultrasonic-assisted extraction (UAE) for 20 minutes. It was obvious that MAE took up half time, and would provide higher efficiency for extraction.
|
|---|
