Effects of propofol on glutamate release from rat cerebral cortex nerve terminal
碩士 === 輔仁大學 === 基礎醫學研究所碩士班 === 96 === Propofol is now the most common used intravenous anesthetics for general anesthesia and sedation due to its rapid onset and recovery. Besides the well-known adverse effects of cardiovascular and respiratory depression, recent studies indicate that propofol may c...
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ndltd-TW-096FJU003250022016-05-16T04:09:53Z http://ndltd.ncl.edu.tw/handle/88112284474448395478 Effects of propofol on glutamate release from rat cerebral cortex nerve terminal Propofol對大鼠大腦皮質神經末梢麩胺酸釋放之研究 LU CHENG-WEI 陸正威 碩士 輔仁大學 基礎醫學研究所碩士班 96 Propofol is now the most common used intravenous anesthetics for general anesthesia and sedation due to its rapid onset and recovery. Besides the well-known adverse effects of cardiovascular and respiratory depression, recent studies indicate that propofol may cause excitatory phenomena such as myoclonus, and epilepsy. However, the mechanisms of theses excitatory effects of propofol have not been elucidated. Considering glutamate is the principle excitatory neurotransmitter in the central nervous system and excessive glutamatergic synaptic transmission can cause epilepsy, we examined the effect of propofol on the release of glutamate from rat cerebral cortex nerve terminals (synaptosomes). Results showed that low dose propofol facilitated 4-aminopyridine (4-AP) but not KCl- or ionomycin-evoked glutamate release from nerve terminal. The facilitation of 4-AP-evoked glutamate release by propofol also occurred in the calcium chelation and significantly attenuated by glutamate transporter inhibitors, DL-TBOA and L-trans-PDC. In addition, propofol increased 4-AP-evoked depolarization of the plasma membrane potential. Furthermore, protein kinase C (PKC) inhibition suppressed propofol-mediated facilitation of glutamate release. These results suggest that low dose propofol facilitates glutamate release from rat cerebrocortical glutamatergic terminals by increasing nerve terminal excitability, likely through the activation of PKC pathway. This finding may provide an explanation for subanesthetic propofol induced excitatory phenomena. WANG SU-JANE CHIANG HAN-SUN 王素珍 江漢聲 2008 學位論文 ; thesis 50 zh-TW |
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碩士 === 輔仁大學 === 基礎醫學研究所碩士班 === 96 === Propofol is now the most common used intravenous anesthetics for general anesthesia and sedation due to its rapid onset and recovery. Besides the well-known adverse effects of cardiovascular and respiratory depression, recent studies indicate that propofol may cause excitatory phenomena such as myoclonus, and epilepsy. However, the mechanisms of theses excitatory effects of propofol have not been elucidated. Considering glutamate is the principle excitatory neurotransmitter in the central nervous system and excessive glutamatergic synaptic transmission can cause epilepsy, we examined the effect of propofol on the release of glutamate from rat cerebral cortex nerve terminals (synaptosomes). Results showed that low dose propofol facilitated 4-aminopyridine (4-AP) but not KCl- or ionomycin-evoked glutamate release from nerve terminal. The facilitation of 4-AP-evoked glutamate release by propofol also occurred in the calcium chelation and significantly attenuated by glutamate transporter inhibitors, DL-TBOA and L-trans-PDC. In addition, propofol increased 4-AP-evoked depolarization of the plasma membrane potential. Furthermore, protein kinase C (PKC) inhibition suppressed propofol-mediated facilitation of glutamate release. These results suggest that low dose propofol facilitates glutamate release from rat cerebrocortical glutamatergic terminals by increasing nerve terminal excitability, likely through the activation of PKC pathway. This finding may provide an explanation for subanesthetic propofol induced excitatory phenomena.
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WANG SU-JANE |
author_facet |
WANG SU-JANE LU CHENG-WEI 陸正威 |
author |
LU CHENG-WEI 陸正威 |
spellingShingle |
LU CHENG-WEI 陸正威 Effects of propofol on glutamate release from rat cerebral cortex nerve terminal |
author_sort |
LU CHENG-WEI |
title |
Effects of propofol on glutamate release from rat cerebral cortex nerve terminal |
title_short |
Effects of propofol on glutamate release from rat cerebral cortex nerve terminal |
title_full |
Effects of propofol on glutamate release from rat cerebral cortex nerve terminal |
title_fullStr |
Effects of propofol on glutamate release from rat cerebral cortex nerve terminal |
title_full_unstemmed |
Effects of propofol on glutamate release from rat cerebral cortex nerve terminal |
title_sort |
effects of propofol on glutamate release from rat cerebral cortex nerve terminal |
publishDate |
2008 |
url |
http://ndltd.ncl.edu.tw/handle/88112284474448395478 |
work_keys_str_mv |
AT luchengwei effectsofpropofolonglutamatereleasefromratcerebralcortexnerveterminal AT lùzhèngwēi effectsofpropofolonglutamatereleasefromratcerebralcortexnerveterminal AT luchengwei propofolduìdàshǔdànǎopízhìshénjīngmòshāofūànsuānshìfàngzhīyánjiū AT lùzhèngwēi propofolduìdàshǔdànǎopízhìshénjīngmòshāofūànsuānshìfàngzhīyánjiū |
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