| Summary: | 碩士 === 中華醫事科技大學 === 生物科技研究所 === 95 === The polyphenols are contained in colorful fruits, such as Hylocereus polyrhizus. The polyphenols are a group of natural dietary antioxidants, and are useful for health in dietary supplementation. The enzyme monoamine oxidase (MAO) is closely related to neurodegeneration disorders such as Parkinson’s disease, depression, etc. MAO enzyme activity increases during aging in the brain. For the reason, it is regarded as a physiological marker for aging. Moreover, the MAO Inhibitors are often used to treat the neurodegeneration diseases. The major purpose of this research is to build the anti-aging analysis model by MAO enzyme activity evaluation, and the Hylocereus polyrhizus constituents was used in this work. It includes two studies in our research. Firstly, The C6 cell (rat brain neuroglia cell line) was used for MAO enzyme activity assay. The result indicated that Hylocereus polyrhizus extracts demonstrate inhibition capacities on C6 cell MAO activity. The 80 weeks old female BALB/c mice were used as animal model in the following study. Mice were divided into 3 groups: (1) control group (C), (2) Hylocereus polyrhizus extracts low dose supplement group (L) (1g extract/kg diet), (3) Hylocereus polyrhizus extracts high dose supplement group (H) (5g extract/kg diet). The mice were fed the test diets for 7 weeks. These results suggested that MAO activity in brain showed significantly decrease in H group compared with C group. Thiobarbituric acid reactive substances (TBARS) levels were also significantly decreased in H group compared with C group in brain. The catalase activity in liver demonstrated significantly increase in L and H groups compared with C group. However, the catalase activity in brain showed significantly decrease in H group compared with C group. In addition, the SOD activity in brain was significantly increased in L and H groups compared with C group. In conclusion, the inhibition capacity of Hylocereus polyrhizus extracts in MAO enzyme activity has been established by these results of cell culture and animal models. These studies above can provide for the bases of food selections for neurodegenerative prevention.
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