| Summary: | 碩士 === 高雄醫學大學 === 天然藥物研究所 === 96 === Coptosapelta diffusa Steenis (Thysanospermum diffusum Champ., Rubiaceae) is a woody lianas, distributed in Southern China, Ryukyu, and Taiwan in broad-leaved forests. The MeOH extract of its whole plant showed significant antitubercular activity against Mycobacterium tuberculosis H37Rv. and was partitioned into EtOAc, n-BuOH and H2O-soluble parts. Investigation of the EtOAc-soluble part by repeated column chromatography led to the isolation of thirty-four compounds, including fourteen triterpenoids: thysanolactone (1), coptosalactone A (2), coptosalactone B (3), coptosalactone C (4), coptosalactone D (5), coptosalactone E (6), coptosalactone F (7), lupeol (8), lupenone (9), lupane (10), friedelin (11), β-amyrin (12), 3-acetyl ursolic acid (13), squalene (14); one naphthalene: methyl 1,4-dimethoxy-2-naphthoate (15); one benzophenone: 2-carbomethoxy-2''-hydroxy-5''-methyl-benzophenone (16); seven anthraquinones: 2-dimethoxymethylanthraquinone (17), 1-hydroxy-6-methylanthraquinone (18), 2-methylanthraquinone (19), 1-hydroxy-2-methylanthraquinone (20), 2-carbomethoxyanthraquinone (21), 2-carbomethoxy-1-hydroxyanthraquinone (22), 2-formylanthraquinone (23); three naphthoquinones: hydroxyhydrolapachol (24), lapachol (25), dehydro-α-lapachone (26); four steroids: β-sitostenone (27), ergosta- 4,6,8(14),22-tetraen-3-one (28), a mixture of β-sitosterol (29) and stigmasterol (30); one benzenoid: octadecyl ferulate (31); one quinone: α-tocopheryl quinone (32); one diterpenoid: trans-phytol (33); one fatty acid ester: methyl linoleate (34). The structures of these compounds were determined through spectral analysis.
Among these isolates, 2, 4, 5, 6, 7 and 16 are new compounds, and compounds 3, 15, 17, 18 and 24 were first isolated from natural source. Compounds 23, 24, 25 and 26 showed antitubercular activity in vitro.
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