| Summary: | 碩士 === 高雄醫學大學 === 生物醫學暨環境生物學研究所碩士班 === 96 === Covalent attachments of polyethylene glycol (PEG) molecules to drugs, proteins, and liposomes is a Food and Drug Administration (FDA) proven technology to improve the bioavailability, safety and efficacy of these therapeutic agents. Qualitative and quantitative analysis of PEG-derivatized molecules are important for both drug developments and applications. We previously reported the development of an antibody based sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of PEG-modified molecules, but not free PEG. In this study, we constructed a cell line that surface expressed an anti-PEG antibody (AGP3) in order to set up a cell-based ELISA to quantify free PEG in a competitive assay. Owing to its high sensitivity, the cell-based quantitative ELISA can replace the conventional chromogenic method for the detection of free PEG and provides a valuable tool to measure free PEG and PEG-modified molecules in biological fluids (such as serum) in preclinical and clinical studies.
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