The application of surface anti-polyethylene glycol antibody in biomedical research
碩士 === 高雄醫學大學 === 生物醫學暨環境生物學研究所碩士班 === 96 === Covalent attachments of polyethylene glycol (PEG) molecules to drugs, proteins, and liposomes is a Food and Drug Administration (FDA) proven technology to improve the bioavailability, safety and efficacy of these therapeutic agents. Qualitative and quan...
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ndltd-TW-096KMC051140022015-10-13T14:04:51Z http://ndltd.ncl.edu.tw/handle/82820647956812077060 The application of surface anti-polyethylene glycol antibody in biomedical research 細胞膜表現辨認聚乙二醇抗體之應用 Bo-Jyun Huang 黃柏鈞 碩士 高雄醫學大學 生物醫學暨環境生物學研究所碩士班 96 Covalent attachments of polyethylene glycol (PEG) molecules to drugs, proteins, and liposomes is a Food and Drug Administration (FDA) proven technology to improve the bioavailability, safety and efficacy of these therapeutic agents. Qualitative and quantitative analysis of PEG-derivatized molecules are important for both drug developments and applications. We previously reported the development of an antibody based sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of PEG-modified molecules, but not free PEG. In this study, we constructed a cell line that surface expressed an anti-PEG antibody (AGP3) in order to set up a cell-based ELISA to quantify free PEG in a competitive assay. Owing to its high sensitivity, the cell-based quantitative ELISA can replace the conventional chromogenic method for the detection of free PEG and provides a valuable tool to measure free PEG and PEG-modified molecules in biological fluids (such as serum) in preclinical and clinical studies. Tian-Lu Cheng 鄭添祿 2008 學位論文 ; thesis 53 zh-TW |
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碩士 === 高雄醫學大學 === 生物醫學暨環境生物學研究所碩士班 === 96 === Covalent attachments of polyethylene glycol (PEG) molecules to drugs, proteins, and liposomes is a Food and Drug Administration (FDA) proven technology to improve the bioavailability, safety and efficacy of these therapeutic agents. Qualitative and quantitative analysis of PEG-derivatized molecules are important for both drug developments and applications. We previously reported the development of an antibody based sandwich enzyme-linked immunosorbent assay (ELISA) for the detection of PEG-modified molecules, but not free PEG. In this study, we constructed a cell line that surface expressed an anti-PEG antibody (AGP3) in order to set up a cell-based ELISA to quantify free PEG in a competitive assay. Owing to its high sensitivity, the cell-based quantitative ELISA can replace the conventional chromogenic method for the detection of free PEG and provides a valuable tool to measure free PEG and PEG-modified molecules in biological fluids (such as serum) in preclinical and clinical studies.
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Tian-Lu Cheng |
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Tian-Lu Cheng Bo-Jyun Huang 黃柏鈞 |
author |
Bo-Jyun Huang 黃柏鈞 |
spellingShingle |
Bo-Jyun Huang 黃柏鈞 The application of surface anti-polyethylene glycol antibody in biomedical research |
author_sort |
Bo-Jyun Huang |
title |
The application of surface anti-polyethylene glycol antibody in biomedical research |
title_short |
The application of surface anti-polyethylene glycol antibody in biomedical research |
title_full |
The application of surface anti-polyethylene glycol antibody in biomedical research |
title_fullStr |
The application of surface anti-polyethylene glycol antibody in biomedical research |
title_full_unstemmed |
The application of surface anti-polyethylene glycol antibody in biomedical research |
title_sort |
application of surface anti-polyethylene glycol antibody in biomedical research |
publishDate |
2008 |
url |
http://ndltd.ncl.edu.tw/handle/82820647956812077060 |
work_keys_str_mv |
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