Influence of GM6001 on matrix metalloproteinase-9 in cerebrospinal fluid of mice infected with Angiostrongylus cantonensis

• Main Authors: Zong-Sheng Wu, 吳宗聲
• Other Authors: Chuan-Min Yen
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/81483831170841915042

碩士 === 高雄醫學大學 === 醫學研究所 === 96 === Abstract BALB/c mice infected with 30 Angiostrongylus cantonensis L3 larvae and then treated with the mmp inhibitor, GM6001, might develop longer survival days, less total leukocyte counts, less eosinophil counts and less albumin concentration in the cerebral spinal fluid (CSF) and less worms recovered from the brain than those of untreated mice at 30 days after infection. There were significant difference between treated and untreated mice in total leukocyte counts and albumin concentrations in CSF at 30 days after infection (P<0.05), and in the recovery of worms from brain at 12, 18 ,24and 30 days after infection (P<0.05). All groups of mice infected with different numbers of L3 larvae and then treated with GM6001 might develop less total leukocyte counts, less eosinophil counts and less albumin concentration in the CSF and less worms recovered from the brain than those groups of untreated mice at 21 days after infection. There were significant difference between treated and untreated mice in total leukocyte counts and albumin concentration in CSF of mice infected with 40 larvae (P<0.05). Mice began to be treated with EACA from 15 days after infection might develop significantly less worms recovery compared with untreated mice (P<0.05). BALB/c mice, infected with 30 Angiostrongylus cantonensis L3 larvae and then treated with GM6001, developed lower MMP-9 and TIMP-1levels in CSF. There were significant difference between treated and untreated mice in MMP-9 levels on days 18 and 30 (P<0.05), and in TIMP-1 levels at 30 days after infection (P<0.05). In this study, those results indicate that GM6001 could palliate clinical symptoms of angiostrongyliasis mice and infected mice were convalescently well after severe inflammation.