| Summary: | 碩士 === 高雄醫學大學 === 藥學研究所碩士班 === 96 === Colon cancer is the second highest cancer-related cause of death after lung cancer in the US. Because of increasing westernization in life type, it has also become a deadly disease in Taiwan as well. Curcuminoids which is a popular Oriental spice made from the root of tumeric (Curcuma longa), has been reported to be a potent antioxidant and free-radical scavenger. In vitro and in vivo studies have shown its potent antiproliferative and proapoptotic effects. In this thesis research, curcuminoids has been chosen as the model drug, and liposome was used as a biodegradable carrier for curcuminoids. The goal of this study is to develop a site-targeting drug delivery system for enhancing the delivery of anti-cancer drugs to the colorectal cancer, using the liposome techonology.
Four liposome formulations, different in the molar ratio of DMPC and cholesterol was optimized based on liposomal size , entrapment efficiency of curcuminoids, and the cytotoxicity in colorectal cancer. Using five Taiwanese human colon cancer primary culture cells to study the cytotoxicity effect of curcuminoids-encapsulated liposome, and detected by MTT test. The IC50 value of the curcuminoids-encapsulated liposome was found to be lower than free form curcuminoids about three fold. The liposomal encapsulation was also observed with a sustained cytotoxicity effect.
In vitro colon mucosa permeation and colon mucosa partition studies founds that liposomal encapsulation has rendered curcuminoids less permeable through the colon mucosa than free-form curcuminoind, but made curcuminoids accumulated inside the colon mucosa tissue.
The results have led us to conclude that the liposomal delivery system developed could benefit the chemotherapeutics of colorectal cancer by minimizing the systemic distribtuion of anti-cancer curcuminoids via the blood circulation, while enhancing the cytotoxicty of anti-cancer curcumioids
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