Removal of Toxic Compound, Aristolochic Acid, by using Carbopol Material

• Main Author: 葉世銘
• Other Authors: 吳海萍
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/67246684021556516846

碩士 === 明新科技大學 === 化學工程研究所 === 96 === The main purpose of this study is to remove the toxic compound, Aristolochic Acid, existing in Aristolochia liukiuensis. The scientific and safe biomaterial Carbopol is utilized to remove Aristolochic Acid from natural product to nontoxic or less toxic status. Hence the first step for this research is to develope the methodology for analyzing the amount of Aristolochic Acid existing in Aristolochia liukiuensis. A suitable High Performance Liquid Chromatography (HPLC) method with stationary phase of RP-18 (10 μm) and mobile phase of 66% methanol：34% DI water was developed for Aristolochic Acid identification and quantification. Carbopol, which possesses high molecular weight, is cross-linked water-soluble polyacrylic acids polymer. It was used for the purpose of removing toxic compound, since they both are having polar functional groups. The carboxylic acid functional group of Carbopol was identified by Fourier Transform Infrared Spectrometer (FT-IR). Carbopol, which is available in different viscosity grades, has no effect on the biological activity of the drug while has excellent thickening and suspension effects. Therefore Carbopol polymer is also used in this research for making the control-release formulation. Carbopol and the detoxified natural medicine are blended to prepare gelatinic product. Both stability kinetics and the release pattern were tested for the prepared product. The release rate by disintegration is near 100% after five hours but the dissolution rate is only 10% after three hours. The prepared product is stable under room temperature for five days.