| Summary: | 碩士 === 國立中興大學 === 化學系所 === 96 === We report here a simple, inexpensive and sensitive electrochemical detection method of using a disposable barrel plating nickel electrode (Ni-BPE) as biosensors. In this work, a new Ni-BPE/ flow-injection ECD cell is developed and further coupled with HPLC for electroanalysis. The user-friendly design of electrochemical cell is not only suitable for flow injection analysis (FIA) but also attractive in practical applications.
In this study, we demonstrate the electrocatalytic oxidation of amino compounds such as melamine and tranexamic acid by using a disposable barrel plating nickel electrode. First, Melamine has been speculated to add intentionally to raise the apparent protein content of the feedstuff, as the protein concentration is measured by analysis of total nitrogen content. Second, Tranexamic acid is an important reagent in cosmetic skin-whitening formulation and a drug for the inhibition of plasminogen to plasmin in blood. Further electrocatalytic oxidation with FIA and the use of a detector in HPLC for the determination of amino compounds at the Ni-BPE are discussed and investigated. The method was successfully applied to the analysis of the real samples and proved to be suitable for rapid and reliable quality control.
Finally the electrocatalytical activity of Ni-BPE was studied for determination of formaldehyde. Formaldehyde is added inappropriately in food processing for its preservative and bleaching effects. A specifically designed electrochemical cell coupled with high performance liquid chromatography is applied for the determination of Formaldehyde. Under optimized conditions, a linear range from 2 uM—1 mM with a detection limit (S/N = 3) of 0.048 uM was observed. The proposed method was successfully used to detect the amount of Formaldehyde in fish to show its potential use in real applications.
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