Improving extraction efficiency of pesticide residue sample by micron technique

• Main Authors: Chih-Chiang Hu, 胡智強
• Other Authors: 蔡碩文
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/15633131689995115389

碩士 === 國立中興大學 === 食品暨應用生物科技學系 === 96 === Pesticides employment is economical and convenient methods to increase crops output and reduce the production cost in Taiwan where the whether is hot and rainy. In order to ensure the safety of vegetables and fruits, the official agricultural and hygienic units endeavor to control the uses of pesticides and examine the residues on food. The Department of Health, Executive Yuan of Taiwan announced the pesticides multiresidue analytical method (3) in August 2005 which coverd 135 kinds of pesticides. However, in this offical method, homogenization by ordinary blender as sample pretreatment, the recoveries of some pesticides doesn’t perform well. Thiabendazole (TBZ) is one of systemic pesticides and fungicides which commonly used on the vegetables and fruits. The objective of this study is to improve extraction efficiency of pesticide TBZ residue sample by micron technique. The average particle size of the cabbage samples was 44.4 μm ± 6.0 μm after micron technique treatment. Micronized cabbage sample with spiked TBZ was determined by official method. The recoveries of TBZ spiked in micronized cabbage samples is 73.3% ± 3.3%, which is higher than 67.1% ± 2.5% by general method. The recovery efficiency was significantly improved. Bean sprouts were divided into two groups including experimental group and control group to simulate the pratical use of TBZ. In the experimental group, the beans were soaked in 1 μg/mL TBZ for 8 hours before germing. The bean sprouts were collected and processed by micron technique. Results show that TBZ has changed to another compound during growth and it decreased by micronized process. In the future, sample treated by micron technique is suggested to be carefully evaluated for bringing effects on determining target.