| Summary: | 碩士 === 國立成功大學 === 化學系碩博士班 === 96 === Su-Dangshen (Codonopsis tubulosa) and Wun-Dang.(C. pilosula), purchased from Taiwanese market, were individually extracted by water. The water extract was quantitatively analysed by 1H-NMR after manual solid phase extraction (SPE) procedure. Furthermore, two novel alkaloids, codotubulosine A and B were also isolated from C. tubulosa .
Using 1H-NMR spectroscopy, a qualitative and quantitative analysis of codotubulosine A, codotubulosine B, adenosine and 5-(hydroxymethyl) furfural was successfully developed toward each of the 42 samples of Codonopsis herbs which were collected from Taiwan and Mainland China. The result of our analysis reveals that codotubulosine A is the major constituent in C. pilosula, C. pilosula var. modesta and C. tangshen, while codotubulosine A and B were found to be the major constituents in C. tubulosa, but both compounds were not discovered in C. clematidea, C. lanceolota, Campanumoea javanica and C. subglobosa. Up to the present, C. pilosula, C. pilosula var. modesta, C. tangshen and C. tubulosa (Su-Dang.) were available traditional herbs on the market in Taiwan and China. The marker components, codotubulosine A and B, were proposed for qualitative and quantitative analysis of Codonopsis.
A rapid identification of the species of Codonopsis by 1H-NMR spectroscopy using manual solid phase extraction (SPE) as a pre-treatment allowed the quantitative analysis of codotubulosine A, codotubulosine B, adenosine and 5-(hydroxymethyl) furfural.
1H-NMR can be used as an analytical tool, and it provides a rapid, accurate, and convenient method for qualitative and quantitative analysis of alkaloid derivatives from Dangshen. This method can also be applied to the analysis of commercial pharmaceutics or related products as well as dietary supplements.
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