Genomic and proteomic analysis of host response during nodavirus infection in grouper

• Main Authors: Young-Mao Chen, 陳永茂
• Other Authors: Tzong-Yueh Chen
• Format: Others
• Language: en_US
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/67966521682417383565

博士 === 國立成功大學 === 生物科技研究所碩博士班 === 96 === Grouper (Epinephelus coioides) is one of the most important economical fishes in Taiwan, but because virus infection in the adult orange-spotted groupers has often been neglected, thus groupers should have an efficient defense system against the nodavirus, but at the same time the antigens of the nodavirus were also taken noticed of, since the groupers were easily infected by the virus at their juvenile and larvae stage. An outline of it is making use of both the genomic and proteomic techniques during the research to illustrate the interaction between the host and the nodavirus, which caused the immune response of the host after which the groupers were infected by the nodavirus. It has been previously found that an overexpression of grouper Mx was proclaimed as a negative regulator of nodavirus activity through direct interaction, such that grouper Mx induction expression might bind and perturb intracellular localization of coat protein. Deletion analysis of grouper Mx indicated that coat protein of nodavirus could bind to the effector domain of Mx. The presence of grouper Mx in dsRNA poly[I:C] interferon system inhibited nodavirus infection, which showed that overexpressing grouper Mx had an inhibitory effect on both coat protein and RdRp of nodavirus antigens, resulting in reducing levels of virus yields. This study demonstrated a key role of grouper Mx in cellular resistance to nodavirus infection.