Characterize the functional role of CEBPD/Rb inHMDB-induced apoptotic gene transcription

• Main Authors: Pei-Jung Pei, 陳姵蓉
• Other Authors: Wen-Chang chang
• Format: Others
• Language: zh-TW
• Published: 2008
• Online Access: http://ndltd.ncl.edu.tw/handle/83999089937371101128

碩士 === 國立成功大學 === 藥理學研究所 === 96 === The CCAAT/enhancer-binding protein (CEBP) family of transcription factors plays an important role in controlling cell proliferation and differentiation. CEPBD, one of the CEBP family members, has been reported to be a potential tumor suppressor because it can induce growth arrest to involve in differentiation and play as a crucial regulator of pro-apoptotic gene expression. Retinoblastoma protein（Rb）is well known to be an important regulator in the G1/S transition or differentiation through the interaction with E2F1. In this study, we found that a DBM structure-related compound - hydroxymethyldibenzoylmethane (HMDB) can induce CEBPD, PPARG2 and GADD153 transcription. We also demonstrated that Rb physically interacts with CEBPD and plays a negative role in the CEBPD-mediated PPARG2 and GADD153 transcription. Furthermore, our data suggest that CEBPD can activate the apoptotic gene through the de-repression of Rb/E2F1-mediated gene inactivation.